Table 2.
Average Collision Cross Section (Å2), % Sequence Coverage, Electron Capture Efficiency, and Fragmentation Efficiency for Ions of Ubiquitin, Cytochrome c, and Lysozyme
| Protein | Precursor | Avg. ccs (Å2) | % Sequence coverage |
% EffEC | % Efffrag |
|---|---|---|---|---|---|
| Ubiquitin | (ubq+6H)6+ | 1776 | 33 % | 84.3 % ± 0.3 % | 64.7 % ± 1.1 % |
| (ubq+2La)6+ | 1527 | 25 % | 77.2 % ± 0.7 % | 45.9 % ± 0.8 % | |
| (ubq+9H)9+ | 2108 | 59 % | 91.9 % ± 0.6 % | 89.5 % ± 0.6 % | |
| (ubq+3La)9+ | 2014 | 63 % | 93.2 % ± 0.9 % | 85.8 % ± 1.7 % | |
| (ubq+10H)10+ | 2263 | 55 % | 92.4 % | 89.8 % | |
| (ubq+9H+La)12+ | 2526 | 76 % | 93.9 % | 87.5 % | |
| Cytochrome c | (cyt c+8H)8+ | 2472 | 30 % | 87.4 % | 56.9 % |
| (cyt c+La+7H)10+ | 2718 | 53 % | 88.4 % | 66.9 % | |
| (cyt c+12H)12+ | 3000 | 43 % | 89.8 % | 71.8 % | |
| (cyt c+La+11H)14+ | 3194 | 56 % | 94.5 % | 87.7 % | |
| Lysozyme | (lys+10H)14+ | 2692 | - | 72.7 % ± 0.6 % | - |
| (lys+La+7H)10+ | 2692 | - | 74.8 % ± 0.8 % | - |