Fig. 3.

Expression of genes encoding gelatinases in Caco-2 cells as determined by real-time RT-PCR. Comparison of the copy number of a MMP-2 and b MMP-9 mRNAs per 1 μg of total RNA in Caco-2 cells treated with 2.5 mM IP6 and 1 ng/ml IL-1β for 6, 12, and 24 h. In Caco-2, cells did not detect the expression of MMP-9 gene either under normal conditions or following treatment with 2.5 mM IP6 for each interaction time (the results are presented as mean ± SD of three separate experiments; *p < 0.05 vs control Caco-2 cells; #p < 0.05 vs IL-1β-stimulated cells)