Table 1.

Characteristics of primers used in experiment

Gene	Primer sequence	Product amplified (bp)	TM (°C)
MMP1	F: 5′-GCTCATGAACTCGGCCATTCTCTTGGACT-3′	281	81
	R: 5′-CGGGTAGAAGGGATTTGTGCGCATGTA-3′
MMP2	F: 5′-TCCACTGTTGGTGGGAACTCA-3′	121	83
	R: 5′-TGGTCGCACACCACATCTTT-3′
MMP3	F: 5′-CCGAGAAATGCAGAAGTTCCTTGGATTGG-3′	131	82
	R: 5′-TCGGGATGCCAGGAAAGGTTCTGAAGTG-3′
MMP9	F: 5′-TTCTGCCCCAGCGAGAGA-3′	101	81
	R: 5′-GTGCAGGCGGAGTAGGATTG-3′
MMP10	F: 5′ TCTTGCATTCCTTGTGCTGTTG 3′	101	81
	R: 5′ ATTGCTGGGCAAGATCCTTGT 3′
MMP13	F: 5′-ACACCTACACCGGCAAAAGC-3′	121	79
	R: 5′-CATTTGTCTGGCGTTTTTGGA-3′
TIMP1	F: 5′-GCCATGGAGAGTGTCTGCGGATACTTCC-3′	118	86
	R: 5′-GCCACGAAACTGCAGGTAGTGATGT-3′
TIMP2	F: 5′-CCCCAAGCAGGAGTTTCTCGACATCG-3′	100	82
	R: 5′-TGGACCAGTCGAAACCCTTGGAGGCT-3′

bp base pair, TM temperature of melting