Figure 5.

Syt II is contained in CARTS. (A) Syt II cDNA was amplified from HeLa RNA by RT–PCR. Three sets of primers were designed to synthesize cDNA fragments of 486, 499, and 227 bp, respectively, in length from mRNA. (B) Syt II-3 × FLAG was expressed alone (top row) or in combination with GST-PKD2-KD (middle row) or PAUF-MycHis (bottom row) in HeLa cells. The cells were double stained with anti-FLAG and anti-TGN46, anti-GST, or anti-Myc antibodies. The boxed areas are enlarged in the insets. Arrowheads indicate tubules attached to the TGN. Bars, 10 and 5 μm (inset).