Figure 6.

AATF promotes chemo-resistance in vivo. (A) AATF depletion results in a significant increase doxorubicin sensitivity of p53^+/+ tumours. In all, 10⁶ HCT116 cells expressing either control or AATF-specific shRNA were xenograftet into the flanks of NCR^nu/nu mice (n=6 for each group). (B) Immunohistochemical analysis of control and AATF shRNA-expressing tumours excised at the termination point of the experiments shown in (A). Specimens were stained with PUMA-specific antibodies and counterstained with haematoxylin. (C) AATF^TD expression promotes doxorubicin resistance in vivo. p53^+/+ HCT116 cells were transduced to express either AATF^WT, AATF^TA or AATF^TD. In all, 10⁶ HCT116 cells of each group were injected into the flanks of NCR^nu/nu-mice (n=6 for each group). Arrows indicate doxorubicin injections (5 mg/kg, i.p.). Asterisks indicate significance (Student’s t-test, two-tailed, P<0.05).