Table 1.
Comparative bovine primers used for identification of the positive ovine BAC clones in the genome region between MHC Class IIa and IIb*
| Name | Gene symbol | Primer sequence (5’→3’) | Product(bp) | Bovine template sequence |
Positive Ovine BAC clones |
|---|---|---|---|---|---|
| S001 |
VPS52 |
F: ATCAATCAGACGATTCCCAACG |
246 |
UniSTS:279053 |
12 H14;12I12;12 J14; 12 K14;120P21 |
| |
|
R: ATCAGAAACACAAGCTGCTCCT |
|
|
|
| S002 |
ZBTB22 |
F: TCCTACGACTTACTCCCTCC |
250 |
UniSTS:66823 |
12I12;12 J14;258 F9; 289 G18 |
| |
|
R: GGGTCAGGTGGTTGTAGTCT |
|
|
|
| S003 |
KIFC1 |
F: GAGACTGTCCGAGACCTGCT |
1242 |
UniSTS:BV104878 |
170 G9;217 M14;289 G18 |
| |
|
R: CTGTGACTACGCGACGAGC |
|
|
|
| S004 |
Loc100139397 |
F: GGTCATCATGGAGGCAGTCT |
756 |
Exon 6: NC_007324 |
19 H17 |
| |
|
R: CGTTCTCCTAAGCCATATGC |
|
|
|
| S005 |
BAK1 |
F: CATTGCATGGTGCTAACCGA |
293 |
Exon 6: NC_007324 |
None |
| |
|
R: CAAGCTCAGCCTTCCAGAAC |
|
|
|
| S006 |
IHPK3 |
F: ATGTATGAGAGCTTGGCACG |
1000 |
UniSTS:267905 |
212D3 |
| |
|
R: TCAGCTTGTACTCTTCCAGGG |
|
|
|
| S007 |
LEMD2 |
F: ACGTCTACCGCAACAAGCTG |
227 |
ENSBTAE00000168818: Exon 1 |
None |
| |
|
R: GTCTCCGATGTCACCGTAGG |
|
|
|
| S008 |
Loc790333 |
F: GACTGCGAGGTGCCGAAGAA |
776 |
Exon |
94 M24;114B22 |
| |
|
R: GTGGACGGCTACACCTGCAA |
|
|
|
| S009 |
HMGA1 |
F: CTCATGCTCTCATTCGGACA |
625 |
ENSBTAE00000364012: Exon 6 |
57 M5 |
| |
|
R: CAGAACAGGAGGCAATGAGG |
|
|
|
| S010 |
NUDT3 |
F: TGAAGTGGAGAGCCTCACAA |
688 |
ENSBTAE00000213256: Exon 5 |
14E10;300 G8 |
| |
|
R: CTTCTCAGCAGACGATGGAC |
|
|
|
| S011 |
COX5B |
F: GTCTCCGTGGTGCGCTCTAT |
324 |
ENSBTAE00000098033: Exon 1,2 |
130 G21;130 M2;170 K16 |
| |
|
R: GGTGTGGCACCAGCTTGTAA |
|
|
|
| S012 |
PACSIN1 |
F: AAGCCAGCAACAGTAGCAGC |
683 |
ENSBTAE00000336066: Exon 10 |
253I24 |
| |
|
R: TCGTTACCTGGAGACCAAGC |
|
|
|
| S013 |
C6orf106 |
F: AGTGAGCGGCTGAGAGAGTT |
266 |
ENSBTAT00000048861: Exon 1 |
None |
| |
|
R: AACTCGGAGATGAGCACGTC |
|
|
|
| S014 |
SNRPC |
F: CCAATGATGAGACCTCCTGC |
147 |
ENSBTAT00000034155:Exon 6 |
119P19;157 K19;223 N7; 227 J17;232 G24 |
| |
|
R: CAGAGTCACAGCACCATGAT |
|
|
|
| S015 |
TAF11 |
F: TGGATGTGTGTGAGAAGTGG |
561 |
ENSBTAT00000022463: Exon 5 |
194 L19;215 J4;232 G24;234C5 |
| |
|
R: TCATGGTGGAGTATCACAGG |
|
|
|
| S016 |
ANKS1A |
F: CGAGGAATGGCCACAAAG |
894 |
UniSTS:BV105378 |
124P23;320A1 |
| |
|
R: ATCGGTCTTGCCAAACAAAG |
|
|
|
| S017 |
TCP11 |
F: ATCAGCGGATCCACTTGTTC |
373 |
ENSBTAT00000022467: Exon 11 |
24D11 |
| |
|
R: CTGGAGCTCACACACGAGGT |
|
|
|
| S018 |
DEF6 |
F: ACCACCAGCAGCTCCTTCAC |
496 |
ENSBTAT00000036152: Exon 11 |
21 M13;66I6;124 K16; 193E6;206 L10 |
| |
|
R: CCTGGCTTGCTTGTTGACTC |
|
|
|
| S019 |
PPARD |
F: GTTCCATGGTCACCTTCTCC |
353 |
ENSBTAT00000023319: Exon 8 |
28D20;152A4 |
| |
|
R: CCGTGAATCTCGCTTCTCTT |
|
|
|
| S020 |
TEAD3 |
F: CCCATCACAGCTGGATTTTA |
145 |
UniSTS:180986 |
None |
| |
|
R: AAATGAAGTACTGTGCCCCC |
|
|
|
| S021 |
Loc540812 |
F: TGCACTGCAACTTCCTGAAC |
263 |
Exon |
95D10;119O20;158O6 |
| |
|
R: GCACTGCAGGCTGACTATGA |
|
|
|
| S022 |
SRPK1 |
F: CAGACACTTACAGGACGTGG |
273 |
ENSBTAT00000022396: Exon 11 |
269D12;285I5 |
| |
|
R: TGAAGACTGGCACATCATGG |
|
|
|
| S023 |
SLC26A8 |
F: ACATCAGCACCGTCAGTCACC |
222 |
UniSTS:476830 |
26A21;121O15 |
| |
|
R: AGGCGATAGAGGACAAACCACAC |
|
|
|
| S024 |
MAPK14 |
F: GAATGGATAACAAAACACTT |
196 |
UniSTS:279403 |
26A21;121O15 |
| |
|
R: AGGCGATAGAGGACAAACCACAC |
|
|
|
| S025 |
MAPK13 |
F: AGAAGCTCAATGACAAGGCG |
606 |
UniSTS:269171 |
121O15;154 M16 |
| |
|
R: TTCCATTCGTCCACTGTGAG |
|
|
|
| S026 |
BRPF3 |
F: GACGCCTGCATCGTATTAGC |
575 |
ENSBTAT00000017711: Exon 1 |
154 M16;250 L24; 278B11;281D9;300 J5 |
| |
|
R: AGCCAGGTTGCAGATGTCAC |
|
|
|
| S027 |
PNPLA1 |
F: TCCTGAACGCTGTCAACCGA |
449 |
ENSBTAT00000055658: Exon 7 |
78 M7;153 F9;268E18; 319O4;337 K13 |
| |
|
R: CAGGTGGCTGTGCAGGTGAT |
|
|
|
| S028 |
Loc790226 |
F: CCATGACTCCGTAGACAAGA |
483 |
Exon |
3O16;9 G2;9 G3;9 H8; 10 N2;15B13;26D1 |
| |
|
R: ACTGCCATAGCTACTGCTGC |
|
|
|
| S029 |
KCTD20 |
F: CGATGCAATCACTAAGCTGG |
834 |
ENSBTAT00000027439: Exon 8 |
None |
| |
|
R: GCAGTTCTCATCCTTCGCAC |
|
|
|
| S030 |
RPS4Y1 |
F: TGCCAGCCTCTTGTCTCTCT |
430 |
ENSBTAT00000036142: Exon 2 |
2A3;11 H24;63 N7; 82 N20;97O2;120P24 |
| |
|
R: TACACCTGAGGAGGCCAAGT |
|
|
|
| S031 |
CDKN1A |
F: GGATCGCTAAGAGCCGGACA |
861 |
ENSBTAT00000011001: Exon 3 |
None |
| |
|
R: GGCAGTCGCTGCTTGAGGTA |
|
|
|
| S032 |
PPIL1 |
F: AATGGTCAATGCGCCTGCTT |
888 |
ENSBTAT00000003071: Exon 4 |
30O17;139 K9;198 M20;271C5 |
| |
|
R: CACCAACGGCAGCCAGTTCT |
|
|
|
| S033 |
PI16 |
F: CCTAGCAACAGAAGCCTCAA |
461 |
ENSBTAT00000002703: Exon 5 |
54O24 |
| |
|
R: AGGCCAAGATCTCACTGCAA |
|
|
|
| S034 |
FGD2 |
F: CACCTTGGTGACCAACATTC |
414 |
ENSBTAT00000018834: Exon 16 |
304 K7;318I17 |
| |
|
R: ACTGCCATAGCTACTGCTGC |
|
|
|
| S035 |
PIM1 |
F: AAGCACGTGGAGAAGGACCG |
490 |
UniSTS:463218 |
None |
| |
|
R: GACTGTGTCCTTGAGCAGCG |
|
|
|
| S036 |
TBC1D22B |
F: CTGTCCACCACTCCATGTCT |
539 |
ENSBTAT00000018938: Exon 13 |
5 K4;26A20;49B1;98 G9 |
| |
|
R: GGACATTCGGACGTGTAACT |
|
|
|
| S037 |
RNF8 |
F: TCTGAATGGTGTCTGGCTGA |
708 |
ENSBTAT00000010959: Exon 3 |
None |
| |
|
R: TTCTCGAGCTGCTCCACTCT |
|
|
|
| S038 |
Loc509620 |
F: AGTGGCACACCGAAGCTC |
666 |
UniSTS:267349 |
25P1;103D16;207 L11; 271 M7 |
| |
|
R: AACTTCCTCTTGAAGCTTTTGC |
|
|
|
| S039 |
C23H6orf129 |
F: GGCAAGAGAACCGCAAGAAC |
281 |
ENSBTAT00000016009: Exon 4 |
25P1;103D16 |
| |
|
R: GCACGAAGTCCTTCTGGAGC |
|
|
|
| S040 |
MDGA1 |
F: TCTTGGCGTTGCAGAGATGA |
228 |
ENSBTAT00000047505: Exon 16 |
None |
| |
|
R: TGTGCGTGTGTCGAACAACC |
|
|
|
| S041 |
ZFAND3 |
F: CGATTGGTTTAATTTTTTTTTTCA |
200 |
UniSTS:34520 |
159 K21;185 L24;235B3 |
| |
|
R: TGTGAAGTTTGTTAAATGTAAGGAA |
|
|
|
| S042 |
BTBD9 |
F: GATAGGTCTTACGCTGTTAG |
155 |
UniSTS:279369 |
None |
| |
|
R: GAATGTACAGAATAGAAGTG |
|
|
|
| S043 |
Loc781915 |
F: AACCTCAAGTGCCTCTCCAG |
714 |
Exon |
67D11;70 N21;76E1; 240 K15;240O16 |
| |
|
R: AACAAGTGTAGCCAGCCATC |
|
|
|
| S044 |
GL01 |
F: GATAGGTCTTACGCTGTTAG |
155 |
UniSTS:279369 |
None |
| |
|
R: GAATGTACAGAATAGAAGTG |
|
|
|
| S045 |
Loc525414 |
F: GAAGAAGAGGTGATCGGTGTAGAG |
216 |
UniSTS:476833 |
8 J2;13E21;24 K16; 24 N15;28 L5;112 N3 |
| |
|
R: TTTCTCCTTCCCATACATTTCTGTG |
|
|
|
| S045b |
GLP1R |
F: CGAGTGTGAGGATTCCAAGC |
418 |
Exon 4, 5 and intron |
80 G15;138P3 |
| |
|
R: GTAGCCCACCGTGTAGATGA |
|
|
|
| S046 |
C23H6orf64 |
F: GTCACAGCCACCATGGAGTC |
415 |
ENSBTAT00000001425: Exon 2 |
19 F4;80 G15;138P3; 156B12; 336 L24 |
| |
|
R: CGCAAGCTGTTCTCAGTCAA |
|
|
|
| S047 |
KCNK5 |
F: CTCCGACTCTGTGCTGGTGA |
774 |
ENSBTAT00000014756: Exon 5 |
None |
| |
|
R: TACCACGCCTTGTACCGCTA |
|
|
|
| S048 |
KCNK17 |
F: AGAGTCCAGGCTCCTTCTAT |
493 |
ENSBTAT00000013646: Exon 5 |
None |
| |
|
R: CTGCTATCCTCAGAGTTCCA |
|
|
|
| S049 |
Loc100139627 |
F: GTGGAGGGAACCTGCGGCAC |
344 |
NC_007324.3: designed online |
3 L3;51O8;189 L22; 253I5; 270 L14 |
| |
|
R: AGGCCTCGGAAGAGCCCTGG |
|
|
|
| S050 |
Loc100138924 |
F: CTTGGTCTTGCGGGCCCCTG |
493 |
NC_007324.3: designed online |
145 G9;146 H11 |
| |
|
R: CCAGGCTCTAGCCCTGCCCA |
|
|
|
| S051 |
DAAM2 |
F: CAGGGAGTGCTCTCAAAGGTAAAGG |
307 |
UniSTS:476834 |
None |
| |
|
R: TCCTCCAGCCTGACTTCTCCTTC |
|
|
|
| S052 |
MOCS1 |
F: GGTCCAGGAAGGCTGAAGTG |
661 |
ENSBTAT00000013792: Exon 11 |
None |
| |
|
R: GAAGGACGGATGGCTATGGT |
|
|
|
| S053 |
LRFN2 |
F: TTGTCATACACGGCGGTCCT |
493 |
ENSBTAT00000023907: Exon 1 |
77E2;220 J8;325 J12; 325 J13 |
| |
|
R: AGCTGAGCCTCGACCACAAC |
|
|
|
| S054 |
UNC5CL |
F: TGACCAACGAGCAGCCACAC |
278 |
UniSTS:476835 |
None |
| |
|
R: GCAGCAGGAGGAGCCAGAAG |
|
|
|
| S055 |
NFYA |
F: GCCGATGAAGAAGCTATGAC |
550 |
ENSBTAT00000013080: Exon 10 |
76 K24;118P22;136B19 |
| |
|
R: CATGAGATGGAGCTTCCTTG |
|
|
|
| S056 |
TREM2 |
F: ACAACTCCTTGAAGCACTGG |
229 |
ENSBTAT00000009568: Exon 2 |
86A4;178 L4;208 M19; 282 F4 |
| |
|
R: TGGAGGCTCTGGCACTGGTA |
|
|
|
| S057 |
TREM1 |
F: CATCATTCCTGCAGCATGTG |
515 |
ENSBTAT00000023397: Exon 4 |
30C8;73 K17;75A11; 75I21 |
| |
|
R: GGCTGTGCCAGGTCTTAGTT |
|
|
|
| S058 |
LOC783024 |
F: CTGAGGACCAAGGCCATGCT |
216 |
Exon |
None |
| |
|
R: TGGTGTGGCACTGCAGGAAG |
|
|
|
| S059 |
FOXP4 |
F: AATTATCGCTCCAAGAGATTCCAC |
250 |
UniSTS:384935 |
112I1;144 K17;181 F9; 299P14;314 F18 |
| |
|
R: CCCATCCTTGTCTCCTCTTTACAT |
|
|
|
| S060 |
MDFI |
F: GCTGTGTCCACTGCATCTTG |
256 |
ENSBTAT00000025763: Exon 4 |
70B14;166C6;181 J11; 202B12; 229A10 |
| |
|
R: GGTCAGGAGGAGAAGCAGAG |
|
|
|
| S061 |
PGC |
F: GAAATTCTCTGCTAAACCCCTTCA |
268 |
UniSTS:385581 |
14 G18;24O7;24O10; 103 G9; 139 N14 |
| |
|
R: TCATCTAAGCAGAAACACCAGTAAATG |
|
|
|
| S062 |
USP49 |
F: GATGGAGTTCATGTAGCAGGTGTT |
260 |
UniSTS:385828 |
None |
| |
|
R: GGAGCGCAAGAAGGAGGAG |
|
|
|
| S063 |
BYSL |
F: TCAGAGGACCTGGAAGTGGA |
538 |
ENSBTAT00000013326: Exon 7 |
3 M12;98 J10;182 F10 |
| |
|
R: CTCTCATGCACAGCAGTGGA |
|
|
|
| S064 |
TAF8 |
F: TGGAGGAAGGAACTTGGTCACAGAG |
228 |
UniSTS:476836 |
103 M11;133 J10;146 L22 |
| |
|
R: GGTGCTTGAGGTTCGTTGAGTTGAG |
|
|
|
| S065 |
MGC137036 |
F: GAAGCAGGACCGTGAGCAGA |
238 |
ENSBTAT00000017035: Exon 2 |
100O15;117E7;133 J9; 146 L22;171 L22;176P6 |
| |
|
R: CTACGAGCGCCACAAGACCA |
|
|
|
| S066 |
TRERF1 |
F: GTGTGTCTGTTGCTGCGGTG |
643 |
ENSBTAT00000020376: Exon 1 |
1O22;17 J12;79 H15; 81 J21;100O15;259 L15 |
| |
|
R: TGGTCTAGGCTTGGCTGTTG |
|
|
|
| S067 |
Loc786000 |
F: TGGCAAGATGGCGGTGCCAG |
379 |
NC_007324.3: designed online |
6P21;32P14;142C8; 162E5;195C23;227D22 |
| |
|
R: AGCAGCCTTGGCCCCACTCT |
|
|
|
| S068 |
UBR2 |
F: CTGCAAGCAACTGACCTCAC |
169 |
ENSBTAT00000007833: Exon 2 |
6P21;129B6;162E5; 163E23;177 M6 |
| |
|
R: CCAACTCAGGATCTTCACCA |
|
|
|
| S069 |
PRPH2 |
F: GTAGTGGACTCCAGGAACTTCG |
232 |
UniSTS:279013 |
26 J6;26 L8;29 M14; 127A7;134B12;177A2 |
| |
|
R: ACCACAGAGTCACCTGCTGAGA |
|
|
|
| S070 |
Loc540169 |
F: ATGAAAGGGTCAGGCGAAC |
130 |
UniSTS:94727 |
144A13;164 L3;164 M2;164 M3;172O18;185 N10 |
| |
|
R: ACAGAGCCGCTAACCGTG |
|
|
|
| S071 |
CNPY3 |
F: GAACAGTGGTCTGGCAAGAA |
214 |
ENSBTAT00000021132: Exon 10 |
98 J16;172O18;185 N10;189O8;289 J21 |
| |
|
R: GTTAGGCTCAGAGCTCGTCA |
|
|
|
| S072 |
CUL7 |
F: TTTCGACCTCGCTCTGAGTT |
1,000 |
UniSTS:270008 |
74C2;189O8;289 J21; 325 K12 |
| |
|
R: CTCCAGCATGTGCCAGTG |
|
|
|
| S073 |
PTK7 |
F: GACTCAGGAGCCTTCCAGTG |
531 |
UniSTS:268417 |
54A6;127D14;142 L8; 163O23;204P7 |
| |
|
R: CTGTATTGCAGCTTCCGAGG |
|
|
|
| S074 |
Loc540077 |
F: CTGAATACCTGATCCGATGG |
417 |
Exon |
54A6;142 L8;163O23; 204P7 |
| |
|
R: GCATGTGCATGAGTAGGTCC |
|
|
|
| S075 |
Loc786439 |
F: GGCGTCTTTAATCAGGATTTGG |
200 |
UniSTS:222501 |
None |
| |
|
R: AATCCAACACTTGAAACCGACA |
|
|
|
| S076 |
ZNF318 |
F: CTGTCTTCACTCGAAGCTCC |
438 |
ENSBTAT00000013481: Exon 1 |
24 L23;66 G8;83 N5; 119 J9;162 F10 |
| |
|
R: AGCTCCTACTTCGTTCCTCC |
|
|
|
| S077 |
TJAP1 |
F: GAGGACGAGGAAGAGCTGAA |
654 |
ENSBTAT00000035977: Exon 12 |
None |
| |
|
R: CGTGCAGAGGATTGAAGGAG |
|
|
|
| S078 |
POLH |
F: GACAGCCACACACATAAGCA |
497 |
ENSBTAT00000007900: Exon 11 |
68 F17;71 H18;74P6; 124 L6;250 J4 |
| |
|
R: GTCTCACAGAGTCGGACACG |
|
|
|
| S079 |
MRPS18A |
F: AGTCGTGAGACCACTGCAGC |
191 |
ENSBTAT00000056429: Exon 6 |
115P10;176 M14; 233 H10;278 K6;291I13 |
| |
|
R: AGGACCTCCTGAGAGCCTGA |
|
|
|
| S080 |
VEGFA |
F: GATCATGCGGATCAAACCTCACC |
326 |
UniSTS:471318 |
12B17;12 H11;30 L7; 63B18;124 J8;249D14 |
| |
|
R: CCTCCGGACCCAAAGTGCTC |
|
|
|
| S081 |
MRPL14 |
F: TCAGAACTGCTCCATTCACG |
182 |
UniSTS:64809 |
117 J15 |
| |
|
R: CAACAACGTGGTCCTCATTG |
|
|
|
| S082 |
SLC29A1 |
F: GGTGGTCTTTGAGCACGACT |
537 |
UniSTS:207086 |
None |
| |
|
R: CCGGAACAGGAAGGAGAAG |
|
|
|
| S083 |
AARS2 |
F: CACTGGAAGCACTGCTGACC |
325 |
ENSBTAT00000018232: Exon 22 |
None |
| |
|
R: GCAGCCAGAACAGCCATGTA |
|
|
|
| S084 |
CDC5L |
F: CCAACTCAGTGGAGGACCAT |
750 |
UniSTS:267825 |
134E15;147I12 |
| |
|
R: GGCTTTGTTTCTGGATTTGG |
|
|
|
| S085 |
SUPT3H |
F: CTTCTGCCTGGAACTTGCACTTG |
208 |
UniSTS:476839 |
23P23;80P15;110 F4;5;6 |
| |
|
R: TGCTTACTGTCTCCCACCTAGATTG |
|
|
|
| S086 |
Loc536911 |
F: TACCAGCCACCGAGACCAA |
309 |
UniSTS:280406 |
9 G19;9 H22;9I23;24; 59B8 |
| |
|
R: AGAGGCTGTTTGACGCCATAG |
|
|
|
| S086b |
CLIC5(BM1258) |
F: GTATGTATTTTTCCCACCCTGC |
158 |
UniSTS:56663 |
291I15 |
| |
|
R: GAGTCAGACATGACTGAGCCTG |
|
|
|
| S087 |
ENPP4 |
F: GAACCAGCTCACCAATGTGT |
595 |
ENSBTAT00000004547: Exon 2 |
72 M13;74O6;127 F7; 182 K12;299 N7 |
| |
|
R: TCCTCTGCTTCACCACCTAA |
|
|
|
| S088 |
RCAN2 |
F: TCTTTACTGTCTGAGCCACC |
132 |
UniSTS:69107 |
None |
| |
|
R: TACACTCAGAGCTAGTTTGC |
|
|
|
| S089 |
CYP39A1 |
F: AGGTGATGGTGGCAACTATG |
200 |
UniSTS:15671 |
57E15;181B7;202D23; 213A17;261 M4 |
| |
|
R: CATGTGTCCATAATTTGATTGC |
|
|
|
| S090 |
TDRD6 |
F: GAGTTCTTCCACCTGCCGTC |
490 |
ENSBTAT00000013158: Exon 1 |
114B7;147E14;190 N9; 329 H12;350E16 |
| |
|
R: ATACCTGAGCCATGCTCTCG |
|
|
|
| S091 |
Loc785478 |
F: TACGCCACCTACACACACAC |
439 |
Exon |
65 L20;133 M1;211 N8; 233B22;233O14 |
| |
|
R: GACTGGTAGCTCCTGATCTG |
|
|
|
| S092 |
GPR116 |
F: CACATCCAGTGCTTATTCAT |
302 |
ENSBTAT00000035930: Exon 18 |
291 M9 |
| |
|
R: TAGACAGAGAAGTTGGCTTG |
|
|
|
| S093 |
GPR110 |
F: AGTGGACAGATACCGGCTGC |
452 |
ENSBTAT00000028795: Exon 10 |
None |
| |
|
R: AGGTGTGGCCATGTGATGGA |
|
|
|
| S094 |
TNFRSF21 |
F: CAGAGCAGAAGGCACCAAGT |
500 |
ENSBTAT00000047874: Exon 11 |
118P16;351 H10 |
| |
|
R: ATTGTCTGCCTCCTTGGTCC |
|
|
|
| S095 |
LOC785024 |
F: GGTTGTCAAGCCACTCGAAT |
611 |
Exon |
14B7;79 L8;168 N8; 264 L6 |
| |
|
R: CGGAGTATATGGCCAGTGTT |
|
|
|
| S096 |
LOC512926 |
F: AGAGCAGAAGGCACCAAGTC |
437 |
Exon |
27A8;290 J19;351 H10 |
| |
|
R: ACGCTCTGCATCTCATCACA |
|
|
|
| S097 |
CD2AP |
F: TACCACAACACCAACTGCAT |
309 |
UniSTS:278169 |
1 H10;14A2;75 J19; 114B12;151 J21;166 L22 |
| |
|
R: TTACCGGGATCACAGAAACA |
|
|
|
| S098 |
GPR115 |
F: CACAGTGGTGGCAGCAATAA |
490 |
ENSBTAT00000003815: Exon 5 |
None |
| |
|
R: GAATAGAGTGCAATGCCGGT |
|
|
|
| S099 |
OPN5 |
F: CTACATCTGCCTGGCGGTCA |
287 |
ENSBTAT00000021933: Exon 4 |
167I8;228 M7 |
| |
|
R: CATGGCTGCTATGGATCCGA |
|
|
|
| S100 |
MGC148542 |
F: ACATTTTCTCCTTCTTTGGCTCC |
272 |
UniSTS:133880 |
1A19;1B9;140A1; 216D18;319I16 |
| |
|
R: GATAGAGGATGACGACAAATGGC |
|
|
|
| S101 |
Loc785693 |
F: AGCCAGGTAGAGTTCCAATG |
518 |
Exon |
17 K13;75E1;76B22; 103 F21 |
| |
|
R: AGTCTCGGCAGTTACCTTGA |
|
|
|
| S102 |
MUT |
F: AGCAAAGCACATGCCAAAAT |
750 |
UniSTS:279392 |
74 J7;8;86P12;252B10; 255 G2;266O16;313 L2 |
| |
|
R: TTCCCCAGAAGAAAGACAAC |
|
|
|
| S103 |
Loc787783 |
F: GGAATCATCAACCCAGTGAGAAAGC |
269 |
UniSTS:476844 |
255 G2;266O16;274D6; 288I23 |
| |
|
R: CACACGGCGGCAGAAAGAGG |
|
|
|
| S104 |
RHAG |
F: GAATCGATGACCATCCATGC |
470 |
ENSBTAT00000015012: Exon 4,5 |
53D7;173C22;186 L10; 226 G3;4;226 H7 |
| |
|
R: AGAAGGCTGGAACATGCGTA |
|
|
|
| S105 |
Loc100138627 |
F: AATGAATAGTATCCCCAATACCTGC |
150 |
UniSTS:164033 |
None |
| |
|
R: GTCCACAAAACATTCTCCTTTCC |
|
|
|
| S106 |
TFAP2D |
F: TAAGCTTTCGGAGAAACCCA |
1422 |
UniSTS:482175 |
5 K4; 139 L18;230 K5 |
| |
|
R: CAGCAGCAAGACTCTCTGGA |
|
|
|
| S107 |
TFAP2B |
F: TGCATGCTCCCTCCTCTC |
120 |
UniSTS:71657 |
25D11;25 F24;142E22; 161A23;167 J23;189D14 |
| |
|
R: CCTCGTCCAATTATGGTGCT |
|
|
|
| S108 |
Loc100138859 |
F: GGAGCACCACAGTACGTAAG |
561 |
Exon |
None |
| |
|
R: GAGGTGTGCCTGTATTGCTA |
|
|
|
| S109 |
Loc537895 |
F: TTCTCTCAAATGATGAATATGCTTC |
270 |
UniSTS:251053 |
56 J7;86O3;87 H23; 277 G10;277 H11 |
| |
|
R: GGACTATTCTATGCATGCCTCTC |
|
|
|
| S110 |
IL17A |
F: CACTCAGGCTGTATCAATGC |
591 |
ENSBTAT00000002786: Exon 3 |
13B24;74A7;74E17; 164 H22;164I23 |
| |
|
R: CAGCTGTGTCATGTACTCCA |
|
|
|
| S111 |
MCM3 |
F: TGTCCCGATTTGACCTTCTC |
515 |
UniSTS:268664 |
69 G8;168E20;223C7; 263 M23;270P6 |
| |
|
R: GTCATCAGGGCTGAAGTTGG |
|
|
|
| S112 |
PAQR8 |
F: TCTATGTCCTGTCCTCCATC |
447 |
ENSBTAT00000035844: Exon 2 |
102 M1;160 L10 |
| |
|
R: AGAAGAAGTAGGCACTGACC |
|
|
|
| S113 |
TRAM2 |
F: TGTTCTACATCTTCATCGCCA |
630 |
UniSTS:267311 |
13P23;53 J18;92C23 |
| |
|
R: ACCAGATCACCGAGCTGAGA |
|
|
|
| S114 |
TMEM14A |
F: CTACCCAAGAAACACTGTCGC |
286 |
ENSBTAT00000006857: Exon 6 |
2C18;31C1;139B24; 183A23;280 K17 |
| |
|
R: AGAGCATTCTATGAAGCCCG |
|
|
|
| S115 |
ICK |
F: ACGGACTGGATCGCTAAGTA |
627 |
ENSBTAT00000020711: Exon 14 |
2C18;76A8;77 G6; 198C12;199 K7 |
| |
|
R: CAGAACAGCACAGCGGTATT |
|
|
|
| S116 |
GCM1 |
F: AGCTGTCCAACTGCCTCCTG |
363 |
ENSBTAT00000010709: Exon 6 |
141A15;199 K7;230E24; 314I2 |
| |
|
R: TGGGAAGGGGAGAAGTCGTA |
|
|
|
| S117 |
ELOVL5 |
F: CTACAGCCACGAGACAGTTT |
182 |
UniSTS:279336 |
64 N21;82O21;90C20; 127 J19;163 F13 |
| R: GGTTTCAATCATTCTTTCAT |
* The bovine oligo primers were designed along the target bovine genomic sequence at an interval of ~80-160 kb between the two neighbor loci, depending on the availability of the DNA sequence that meet the primer selection criteria. A total of 119 pairs of primers were listed here.