Table 1.
Physical maintenance conditions for the three Dehalococcoides-containing enrichment cultures
| KB-1 | DonnaII | ANAS | |
|---|---|---|---|
|
Cultures |
|
|
|
| Liquid volume |
1.6 L |
5.7 L |
0.4 L |
| Stirred |
No |
Yes |
Yes |
| Temperature |
20-22 °C |
30 °C |
25-28 °C |
| Electron acceptor |
TCE (858 μmol added/L)1 |
PCE (110 μmol added/L)2 |
TCE (278 μmol added/L)3 |
| Electron donor |
Methanol (4.3 mM)1 |
Butyrate (440 μM)2 |
Lactate (25 mM)3 |
| Feeding frequency |
14 days |
2 days |
4-7 days |
| Residence time |
2 years4 |
70-80 days5 |
16-47 days3 |
| Donor loading rate [(μmol/L/d)/(meeq/L/d)] |
0.31/1.84 |
0.22/4.44 |
4.55/54.5 |
| Acceptor loading rate [(mmol/L/d)/(meeq/L/d)] |
0.061/0.37 |
0.055/0.44 |
0.051/0.30 |
| Donor eeq/Acceptor eeq |
5 |
10 |
180 |
| Cobalamin amended |
B12 (0.005 μg/L) |
B12 (1 μg/L) |
B12 (0.1 μg/L) |
| Dhc strain(s) | KB-1/PCE & KB-1/VC1 | DET1955 | ANAS (2 strains) 3 |