Table 3.

Presence of metabolic pathways for the utilization of the electron donor substrates amended to the three enrichment cultures as detected using MG-RAST annotations

	# Pathways complete/partial*			Taxonomic Classification of Pathways
	DonnaII	ANAS	KB-1	DonnaII	ANAS	KB-1
LACTATE (ANAS)
A - Lactate to Acetate via Pyruvate (3 genes)	2/2	1/6	0/1	α-P, Firm/Act, BC	Firm/α-P, BC, β-P, Cya, γ-P, Planc	/γ-P
B – Lactate to Ethanol (4 genes)	2/2	1/5	0/1	α-P, Firm/Act, BC	Firm/α-P, BC, β-P, Fib, γ-P	/Fib
C - L-lactate to Acetate directly (EC 1.13.12.4)	0	0	0
BUTYRATE (DonnaII)
To Acetyl-CoA and Acetoacetate (5–7 genes)	4/8	2/6	1/2	δ-P, Firm, γ-P, β-P/Act, α-P, BC, Cflx, CrenA, EurA, Fib, Fus	δ-P, Firm/Act, α-P, β-P, EurA, Fus, γ-P	Firm/BC, δ-P
METHANOL (KB-1)
A - to Formaldehyde (ECs 1.1.99.8/1.11.1.6/1.11.1.7)	9	6	6	Act, BC, β-P, DT, δ-P, ϵ-P, EurA, Firm, γ-P	BC, β-P, δ-P, EurA, Firm, γ-P	BC, β-P, δ-P, EurA, Firm, γ-P
B - to Methyl-CoM, eventually to coenzyme M (4 genes)	1	1	1	EurA	EurA	EurA

Abbreviations are as follows: Act = Actinobacteria, α/β/δ/ϵ/γ-P = Proteobacteria, BC = Bacteroidetes/Chlorobi group, Cya = Cyanobacteria, Cflx = Chloroflexi, CrenA = Crenarchaeota, Dhc = Dehalococcoides, DT = Deinococcus/Thermus group, EurA = Euryarchaeota, Fib = Fibrobacteres/Acidobacteria, Firm = Firmicutes, Fus = Fusobacteria, Planc = Planctomycetes, Therm = Thermotogae.

*partial – 40% or greater of pathway must be detected, for lactate to acetate conversions, initial step was required to be present.