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ChIP/control ratio as a function of total count for human NFκB data. NFκB marginal ChIP/control ratio against total with bin-width of 100 bp, both in natural log scale. Sizes of the plotting symbols are proportional to the log (10) of the number of reads. Horizontal dash line indicates the NCIS estimate of the normalization factor. Vertical dash line represents the NCIS total count threshold (tw∗).
