Table 1.

Primers used in multiplex PCR

Primer Name	Nucleotide Sequence	Predicted Amplicon Sizea
H. influenzae FWD	5′-CGTATTATCGGAAGATGAAAGTGC-3′	525 base pairs
S. pneumoniae FWD	5′-AAGGTGCACTTGCATCACTACC-3′	484 base pairs
A. otitidis FWD	5′-GGGGAAGAACACGGATAGGA-3′	264 base pairs
M. catarrhalis FWD	5′-CCCATAAGCCCTGACGTTAC-3′	237 base pairs
Universal REV	5′-CTACGCATTTCACCGCTACAC-3′

^aWhen the designated FWD primer is used in conjunction with the Universal REV primer.