Figure 3.

Effect of TRABID inhibitors on Wnt/β-catenin-mediated transcriptional activity. A,B) TRABID inhibitors fail to inhibit Wnt reporter gene activity. HCT-116 or SW480 cells were transfected with FOPFLASH or TOPFLASH and a plasmid expressing Renilla luciferase and incubated with the compounds for 24 hours. As a control the cells were also transfected with an β-catenin siRNA (siCTNNB). Normalized Wnt reporter gene activity is shown. The experiments were repeated three times in triplicates C) Effect of TRABID inhibitors on endogenous Wnt target gene expression in SW480 cells. SW480 cells were incubated with the compounds (20 μM) for 24 hours, and relative AXIN2 and c-Myc mRNA levels were determined by qRT-PCR normalized on the levels of β-actin mRNA. Experiments are performed in triplicates, and data are presented as means ± SD.