Strains
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E. coli JM109
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recA1, endA1, gyrA96, thi-1, hsdR17, supE44, relA1, Δ(lac-proAB)/F’[traD36, proAB+, lacIq, lacZΔM15]
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TransGen Biotech
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BSUL03
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ΔamyE::(P43::kivd-adh2), P43::ilvD-ilvC-alsS; Spcr, Emr
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[6]
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BSUL04
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BSUL03 with lactate dehydrogenase inactivation (Δldh); Spcr, Emr, Kmr
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This study
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BSUL05
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BSUL04 with pyruvate dehydrogenase complex E2 subunit inactivation (ΔldhΔpdhC); Spcr, Emr, Kmr, Tetr
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This study
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Plasmids
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pUC18
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E. coli cloning vector; Ampr
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Laboratory stock
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pDK
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B. subtilis integration vector with amyE locus; Ampr, Kmr
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BGSC [50]
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pHY300PLK
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E. coli-B. subtilis cloning vector; Ampr, Tetr
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Takara
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pUCL
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pUC18 containing ldh fragment of B. subtilis, Ampr
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This study
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pUCLKm
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pUCL containing kanamycin resistance cassettes from pDK; Ampr, Kmr
|
This study
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pUCP01
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pUC18 containing homology arm pdhC1 fragment of B. subtilis; Ampr
|
This study
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pUCP02
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pUCP01 containing homology arm pdhC2 fragment of B. subtilis; Ampr
|
This study
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pUCPTet |
pUCP02 containing tetracycline resistance cassettes from pHY300PLK; Ampr, Tetr
|
This study |