Table 3.
q-RTPCR confirmation of LT-induced genes.1
| Probe | Microarray | q-RT-PCR | Gene name | Primer sequence |
|---|---|---|---|---|
| 38290_at |
5.61 |
7.40 |
RGS14-F |
CAGGGATCTGTGAGAAACGAG |
| |
|
|
RGS14-R |
AGGTGATCCTGTTTTCCAGC |
| 207008_at |
5.04 |
7.50 |
IL8RB-F |
GTCTAACAGCTCTGACTACCAC |
| |
|
|
L8RB-R |
TTAAATCCTGACTGGGTCGC |
| 210166_at |
3.90 |
2.24 |
TLR5-F |
TTTTCAGGAGCCCGAGC |
| |
|
|
TLR5-R |
AGCCGAGATTGTGTCACTG |
| 212686_at |
2.65 |
3.85 |
PPM1H-F |
GAGTACAGAGAAAGGAGCTTGG |
| |
|
|
PPM1H-R |
TCCAATAGTTGCCATTACCCG |
| 226016_at |
2.38 |
1.60 |
CD47-F |
TTTGCTATACTCCTGTTCTGGG |
| |
|
|
CD47-R |
TGGGACGAAAAGAATGGCTC |
| 209269_at |
2.15 |
1.60 |
SYK-F |
CAAGTTCTCCAGCAAAAGCG |
| |
|
|
SYK-R |
CATCCGCTCTCCTTTCTCTAAC |
| 206991_at |
-2.66 |
-2.33 |
CCR5-F |
CCAAAAGCACATTGCCAAACG |
| |
|
|
CCR5-R |
ACTTGAGTCCGTGTCACAAGCC |
| 205403_at |
-12.5 |
-28.0 |
IL1R2-F |
TGGCACCTACGTCTGCACTACT |
| IL1R2-R | TTGCGGGTATGAGATGAACG |
1 Calculated fold changes comparedtomock treated samples.