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Genotyping and expression analyses of Cdk2ap2tr/tr mESCs. (A) PCR genotyping of various mESC clones with LoH of the Cdk2ap2 locus after high G418 selection. The lower band shows presence of the neoR allele, and the upper band indicates endogenous Wt Cdk2ap2 allele. (B) Northern blot analyses for Cdk2ap2 expression. Lower panel, ethidium bromide-stained gel showing equal loading. mESCs, mouse embryonic stem cells; PCR, polymerase chain reaction; LoH, loss of heterozygosity.
