FIG. 2.

Spontaneous differentiation phenotype in Cdk2ap2^tr/tr mESCs. (A) Deletion of Cdk2ap2 resulted in an inability of the mESCs to grow in defined colonies with smooth borders that are characteristic of mESCs (upper panel). Instead, the Cdk2ap2^tr/tr mESCs grew in a monolayer with a distinctly visible cytoplasm compared to the Wt cells where the nucleus and cytoplasm are indistinct. (B) Colony formation assay was performed by comparing Wt and Cdk2ap2^tr/tr mESCs. Cdk2ap2^tr/tr mESCs had significantly greater differentiation compared to the Wt colonies.