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Generation of interspecific chimeras by injection of Rosa26-tdTomato knock-in rat ESCs into mouse blastocysts. (A) Embryonic day (E) 13.5 interspecific chimera generated by injection of BLK-RT2 rat ESCs into a mouse blastocyst. (B) FACS patterns obtained using embryonic fibroblasts established from an interspecific chimera and stained with a specific antibody against mouse or rat CD54. As a control, mouse embryonic fibroblasts were also analyzed.
