Table 1.
Efficiency of Gene Targeting in Various Types of Rat Pluripotent Stem Cells
| Name | Strain | Cell type | Reference | Transduced cells | Drug- resistant colonies | Picked- up colonies | Targeted clones (%)a |
|---|---|---|---|---|---|---|---|
| BLK2i-1 | DA×Wistar | ESC | [18] | 5×10e6 | 3 | 2 | 1 (50) |
| 5×10e6 | 2 | 2 | 2 (100) | ||||
| 2.5×10e6 | 18 | 12 | 6 (50) | ||||
| DA3i-1 | DA | ESC | 5×10e6 | 48 | 24 | 9 (38) | |
| BN2i-4 | BN | ESC | [5] | 5×10e6 | 8 | 8 | 2 (25) |
| 5×10e6 | 13 | 9 | 2 (23) | ||||
| T1-3 | Wistar | iPSC | [4,12] | 4×10e6 | 9 | 8 | 3 (38) |
| DAT3-1 | DA | iPSC | 5×10e6 | 6 | 6 | 1 (17) | |
| Total | 107 | 71 | 26 (37) |
a
Judged by PCR.
ESC, embryonic stem cell; iPSC, induced pluripotent stem cell; PCR, polymerase chain reaction.