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. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: Dev Cell. 2012 Jul 17;23(1):82–96. doi: 10.1016/j.devcel.2012.04.021

Figure 1. hbs loss and gain-of-function displays PCP eye phenotypes.

Figure 1

(A–F) Tangential eye sections of adult eyes of indicated genotypes, centered around the equator; bottom panels: schematic representations of ommatidial polarity. Anterior is left and dorsal up in all panels. Black and red arrows represent dorsal and ventral chiral forms, respectively, green arrows represent R3-R3 symmetrical clusters, open circles represent ommatidia with loss of R7, black dots represent ommatidia with a reduced number of R-cells.

(A) Wild-type: regular ommatidial arrangement of opposing chiralities forming a mirror image across the equator

(B) sevGal4, UAS-hbs-IR at 25°C: note symmetrical clusters (green arrows), rotation defects, and some loss of R-cells.

(C) sevGal4, UAS-hbs-IR is enhanced by hbs heterozygosity (sev>hbs-IR; hbs459/+), confirming specificity of RNAi.

(D) sevGal4, UAS-hbs-IR at 29°C: note stronger defects as compared to 25°C.

(E) hbs459/Df(2R)ED2423: note PCP defects and R-cell loss comparable to the hbs-RNAi experiments, compare to also (D) for similarity to UAS-hbs-IR phenotypes. Quantified in Table 1.

(F) Overexpression of Hbs using sevGal4 (sevGal4, UAS-Hbs) causes similar eye phenotypes (compare to C-E).

See also Suppl. Figure S1.