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. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: Dev Cell. 2012 Jul 17;23(1):82–96. doi: 10.1016/j.devcel.2012.04.021

Figure 7. hbs genetically interacts with dAppl.

Figure 7

(A-C) Tangential sections of adult eyes of indicated genotypes, anterior is left and dorsal up.

(A) sevGal4, UAS-hbs-IR eye at 25°C with phenotypes as seen previously (e.g. Figure 1B); (B) sevGal4, UAS-appl-IR eye at 25°C, note several ommatidia that adopt R3-R3 type symmetrical arrangement; (C) sevGal4, UAS-appl-IR, UAS-hbs-IR at 25°C. Note enhancement of either inidividual RNAi phenotype, indicating a positive (synergistic) relationship between Appl and Hbs. (D) Quantification of R3-R3 symmetrical clusters in the genotypes shown in A-C. Error bars represent standard deviations (*p<0.01).

(E-G) Confocal projections of third instar larval eye discs expressing the APPLV-GFP fusion protein under the control of sevGal4 (sevGal4>AppLV-GFP) showing expression of GFP behind the MF and its quantification (G). Expression of the GFP reporter is significantly reduced in eye discs co-expressing hbs-IR (sevGal4>AppLV-GFP, UAS-hbsIR), indicating that there is reduction of APPL-ICD membrane cleavage when Hbs is compromised (F). Eerror bars show standard deviations, with *p<0.01 in (G). See also Suppl. Fig. S6F for schematic of reporter assay. See also Suppl. Figure S6.