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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Cell Mol Bioeng. 2012 Feb 8;5(2):194–204. doi: 10.1007/s12195-012-0221-3

Figure 5. Use of ultrathin T-gelatin hydrogel scaffolds in static cell culture.

Figure 5

A. Left: The fully assembled cell culture system seeded with opposing cell monolayers which is optically transparent. Note that the T-gelatin scaffold is taut and does not sag when hydrated and seeded with cells. Right: Light micrograph images of both monolayers (podocyte and endothelial); both images are the same field but in different focal planes. These images appear slightly unfocused due to the optical diffraction caused by the close proximity of the two cell monolayers and the use of transmitted light at low power (10×) magnification. B. Confocal images (XYZ plane) of cell monolayers; all images are the same field, but different focal planes (63× magnification). C. Confocal images (XZY plane) through the height (z plane) of a cell culture system showing scaffold thickness.