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Limiting dilution series of 107–100 copies DNA per reaction; NTC: no template control (a) A. precatorius DNA and (b) R. communis (cultivar Carmencita pink) DNA. For further use as positive control for verification of assay performance quantified PCR products were adjusted to result in final crossing point values of 30 and dried down in a SpeedVac vacuum centrifuge for long term storage.
