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A) Talin-1 and talin-2 were both knocked down in HGFs, plated on FN in the presence or absence of IL-1β for 60 min, and fixed and immunostained for activated β1-integrin (12G10). B) Mean± sd numbers of focal adhesions were computed with MetaMorph. C) NIH 3T3 cells treated with siRNA to kindlin-2 were spread on FN with vehicle or IL-1β for 60 min, fixed, and immunostained for activated β1-integrin (9EG7). D) Metamorph was used to quantify mean ± sd numbers of focal adhesions per cell.
