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. 2012 Nov;26(11):4429–4444. doi: 10.1096/fj.12-207381

Figure 6.

Figure 6.

A) FAK−/− and FAK+/+ mouse embryonic cells were attached overnight to FN and treated with vehicle or with IL-1β (20 ng/ml) in 2% serum-containing medium for 15, 30, or 60 min. β1-Integrin activation was assessed by immunostaining with the 9EG7 neoepitope antibody and quantification of whole-cell fluorescence by flow cytometry. B) Proteins were separated from cell lysates of the various cell types under study and immunoblotted for the indicated proteins.