Table 4.

Nucleotide sequences at the junction of the endogenous gene and vector DNA

Cell line (gene)	Method	Inserted DNA sequence and vector sequence (underscored)
A61 (Btbd9)	Inverse PCR (5′ side)	CGCACACTTTGGGAGAGTAAGCTT–GATATCGAATTCTACCGGG
	Inverse PCR (3′ side)	TACAGGTGGGGTCTTTCA–ACCTAAAAAAGAGAACCTTGCATC
P173 (Crlf3)	5′ RACE	GGCTGCGGGAGGCGCAGAGGCAG–GATGCCTTTGTGGAACTG
	Inverse PCR (3′ side)	TACAGGTGGGGTCTTTCA–TGAGGAAGAATTCGGGGAGAGTTT
P207 (Ssbp3)	5′ RACE	GAAGCAAAAGCCTTTCATGATAC–GATGCTTTGTGGAACTGT
	SPLK PCR	GTCAGCGGGGGTCTTTCA–CTACCTAAAGCCTCTGCCCTGGC
P26 (Btf3l4)	Inverse PCR (3′ side)	TACAGGTGGGGTCTTTCA–GTATGTGATGGTATATTTTTCTTAAT

Vector DNA sequences are underscored. Fig. 4 illustrates insertion sites.