Table 2.
Sphinganine-N17 preferably binds lipid vesicles enriched in sphingomyelin and cholesterol
| Compound |
Ka (M−1) |
|
|---|---|---|
| PC:SM:Chol (1:1:1) | PC:Chol (9:1) | |
| Sphinganine-N17 | 4.7 × 105 ± 1 × 105 | 8.4 × 104 ± 1.4 × 104 |
| C16-N17 | 1.7 × 105 ± 0.3 × 105 | 1.5 × 105 ± 0.7 × 105 |
| Enfuvirtide | 6.4 × 104 ± 1.4 × 104 | 3.1 × 104 ± 0.5 × 104 |
Compound association with lipid vesicles was estimated by titration of NBD-labeled compound with large unilamellar vesicles. Mole ratio between phosphatidylcholine (PC), sphingomyelin (SM), and cholesterol (Chol) is shown for each vesicle. Membrane-binding constants (Ka) were calculated from Eq. 2 in Materials and Methods. Results represent means ± sd of the fitting curve; n = 3.