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. 2012 Nov;26(11):4685–4695. doi: 10.1096/fj.12-212282

Figure 2.

Figure 2.

SNRK increases CacyBP levels and decreases β-catenin protein levels in colon cancer cells. A) Expression and activity of SNRK-GFP and SNRK-T173A-GFP in HCT116 cells; n = 3. B) Levels of CacyBP mRNA in HCT116 cells with overexpression of SNRK or controls; n = 9. C) Levels of CacyBP protein in HCT116 cells with overexpression of SNRK or controls. Top panel: representative Western blots (lanes have been rearranged from the same blot for clarity). Bottom panel: summary of densitometry; n ≥ 3. D) Levels of β-catenin protein in HCT116 cells with overexpression of SNRK or controls. Top panel: representative Western blots. Bottom panel: summary of densitometry; n = 7. E) Levels of β-catenin protein in HCT116 cells with overexpression of CacyBP or vector control (lanes shown are rearranged from the same blot). F) Western blot of SNRK protein in RKO cells treated with SNRK or nonsilencing control (NSC) shRNA. G) Levels of CacyBP mRNA with SNRK down-regulation in RKO cells; n = 10. H) Levels of CacyBP protein with SNRK down-regulation in RKO cells. Top panel: representative Western blots. Bottom panel: summary of densitometry; n = 3. I) Levels of β-catenin protein with SNRK down-regulation in RKO cells. *P < 0.05 vs. GFP and T173A or vs. NSC.