Figure 3.
SNRK decreases colon cancer cell proliferation. A) Proliferation in HCT116 cells transfected with SNRK-GFP, SNRK-T173A-GFP, or GFP, as determined by viable cell counting with trypan blue exclusion at 24, 48, and 72 h; n ≥ 3. B) Proliferation in HCT116 cells after 72 h, as measured by MTS reduction; n ≥ 3. C) Proliferation in RKO cells treated with nonsilencing control (NSC) or SNRK shRNA, as measured by viable cell number at 24, 48, and 72 h; n = 3. D) Proliferation in RKO cells with down-regulation of SNRK at 72 h, as measured by MTS reduction; n = 3. *P < 0.05 vs. GFP and T173A or vs. NSC.