Figure 3.

Effect of known LFA-1 allosteric antagonists or isoflurane on KIM127 and MEM148 epitope exposure. A) KIM127 and MEM148 epitope exposure in the presence of BIRT377 (10 μM) or XVA143 (10 μM). MFI, mean fluorescence intensity. One-way ANOVA with Tukey post hoc analysis was used to compare the data within Mg²⁺/Ca²⁺ or Mn²⁺ group. *P < 0.05 vs. WT. B) KIM 127 and MEM 148 epitope exposure at 2% isoflurane in 1 mM Mn²⁺. *P < 0.05 vs. mock-treated samples; Student's t test. Data are shown as means ± se of 3 independent experiments of triplicates.