Figure 7.

Effect of BIRT377 and TS1/18 on αI and βI mutants. A) Soluble ICAM-1 binding assay. TS1/18 (10 μg/ml) or BIRT377 (10 μM) was used with 1 mM Mg²⁺/Ca²⁺ or 1 mM Mn²⁺. MFI, mean fluorescence intensity. B) KIM127 epitope exposure of β-L132A mutant in the presence of BIRT377 (10 μM). One-way ANOVA with Tukey post hoc analysis was performed. No statistical significance was found between Mn²⁺ and Mn²⁺+BIRT377 of β-L132A. Data are shown as means ± se of 3 independent experiments of triplicates.