Figure 3.

Normal mitochondrial localization of missense SDHB mutant proteins. A) Immunofluorescent detection of SDHB mitochondrial localization. Cells were labeled with anti-Flag (green), Mitotracker (red), and Hoechst33342 (blue). B) Autoradiography of [³⁵S]-methionine labeled SDHB binding and insertion into isolated mitochondria. Liquid scintillation quantification of binding and insertion of mutant SDHB proteins relative to wild-type SDHB. C) Immunoprecipitation assay of SDHA binding to SDHB mutants. D) Quantification of SDHA binding to mutant SDHB relative to wild type SDHB.