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. 2012 Nov;26(11):4506–4516. doi: 10.1096/fj.12-210146

Figure 4.

Figure 4.

Effect of proteostasis modulators on SDHB protein stability. A) Autoradiography of [35S]-methionine-labeled mutant SDHB 4 h after administration of proteostasis modulators LB-201, LB-202, SAHA, celastrol, and quercetin. B) Liquid scintillation quantification of [35S]-methionine-labeled SDHB 4 h after administration of proteostasis modulators. C) Western blot of ΔSDHB-L65P 0–2 h after administration of CHX following treatment with LB-201, SAHA, and celastrol. D) Relative quantity of ΔSDHB-L65P 0–2 h after administration of CHX following treatment with LB-201, SAHA, and celastrol. E) Western blot of ubiquitin and Hsp90 binding to immunoprecipitated ΔSDHB-R49Q following treatment with LB-201, LB-205, and SAHA.