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. 2012 Sep 12;10(9):1955–1976. doi: 10.3390/md10091955

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© 2012 by the authors; licensee MDPI, Basel, Switzerland.

This article is an open-access article distributed under the terms and conditions of the Creative Commons Attribution license (http://creativecommons.org/licenses/by/3.0/).

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Southern blot analysis of genomic DNA from C. zofingiensis. gDNA was digested with ScaI (Lane 1), NdeI (Lane 2), BamHI (Lane 3) or NcoI (Lane 4), electrophoretically separated on a 0.8% agarose gel, blotted and hybridized at high stringency with a probe of 662 bp of the Czzep gene amplified by PCR.