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. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: J Cell Sci. 2006 Sep 12;119(Pt 19):4071–4078. doi: 10.1242/jcs.03192

Figure 3. Stunted is a trimeric molecule.

Figure 3

(A) Sedimentation analysis of 1-1639 and 1-1597. Quantification of a typical experiment where cell lysates were separated on a 15-40% glycerol gradient. Note the second peak at fraction 8 in 1-1639 that is absent in 1-1597.

(B) Sedimentation analysis of Stunted and StuntedΔtripod. Quantification of a typical experiment where cell lysates were separated on a 10-35% glycerol gradient. Note the presence of a second peak in Stunted that is absent in StuntedΔtripod. The sedimentation of molecular weight standards is shown for each gradient (arrows). The calculated molecular weights for monomers of 1-1639, 1-1597, Stunted and Stunted Δtripod were 215, 210, 117 and 108 kDa respectively. Fractions were analysed by SDS-PAGE and immunoblotting for GFP. Following densitometry, the signal in each fraction was expressed as a percentage of the total GFP signal from all fractions on the blot.