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. Author manuscript; available in PMC: 2012 Oct 18.
Published in final edited form as: J Cell Sci. 2006 Sep 12;119(Pt 19):4071–4078. doi: 10.1242/jcs.03192

Figure 5. Constructs containing the N-terminal domain were recruited to the mitotic spindle.

Figure 5

(A) Representative confocal micrographs of each construct in HEK293 cells in metaphase. Scale bar, 10 μm.

(B) A cell expressing 1-1639 on a knockdown background. ROIs (grey, 1.04 × 1.04 μm) were placed over the spindle (black outline) and in the cytoplasm (white outline). Spindle regions and non-spindle areas were defined by staining for α-tubulin using DM1A/Alexa546 (left). Measurements were taken from the GFP channel (right).

(C) Histogram to show the recruitment to the spindle of CHC constructs on a CHC knockdown background. Spindle recruitment is the GFP fluorescence measured in a 1.04 × 1.04 μm ROI at the spindle divided by that measured in a same-sized ROI in the cytoplasm (Fspindle/Fcytoplasm). Results are mean ± s.e.m., from 12-27 cells per construct, **, p < 0.01.