Figure 6. Rescue of mitotic defects by trimeric CHC constructs.
Histograms to show quantification of (A) mitotic index and (B) the frequency of metaphase-like cells with misaligned chromosomes in cells expressing CHC constructs on a CHC knockdown background. Mitotic index was assessed by counting the number of GFP-positive cells in mitosis as a proportion of the total number of GFP-positive cells, per unit area. The frequency of GFP-positive cells in a metaphase-like state with misaligned chromosomes were also counted. H33342 staining was used to identify mitotic cells and misaligned chromosomes. Results are mean ± s.e.m., **, p < 0.01.
