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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Free Radic Biol Med. 2012 Apr 17;52(10):2064–2071. doi: 10.1016/j.freeradbiomed.2012.03.018

Figure 2.

Figure 2

Analysis of nuclear Nrf2 and p-ERK in lung and liver of rats fed organoselenium compounds. (A) Purity of cytoplasmic and nuclear extracts from rat lung were assessed by analyzing levels of GAPDH (cytoplasmic marker) and Lamin A (nuclear marker) of cytoplasmic fractions (lanes 1-4) and nuclear fractions (lanes 5-8) from 4 separate samples of rat lung. Samples were processed and analyzed by Western blot analysis as described in text. Resulting membranes were probed initially with GAPDH antibody (top panel) and later with Lamin A antibody (bottom panel). Relative Nuclear Nrf2 (B) and p-ERK (C) expression in lung and liver of rats fed organoselenium compounds were analyzed. Male F344 rats were fed control diet (AIN-93A) or diets supplemented with p-XSC, p-XSeSG or selenium methionine (10 ppm as selenium) for 2 weeks. Protein expression of nuclear Nrf2 (A) and p-ERK (B), normalized to nuclear Lamin A, in lung and liver was determined by western blotting. Values are mean ± SD (n=6).