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. Author manuscript; available in PMC: 2013 May 15.
Published in final edited form as: Free Radic Biol Med. 2012 Apr 17;52(10):2064–2071. doi: 10.1016/j.freeradbiomed.2012.03.018

Figure 4.

Figure 4

Effects of siNrf2 on Luciferase Activity, GSH and Nuclear Nrf2 in p-XSC or p-XSeSG Treated HepG2 ARE Cells. Cells were grown to 60% confluence then transfected with non-targeting siRNA-Control (siControl) or siRNA-Nrf2 (siNrf2). Thirty hours after siRNA transfection, siControl and siNrf2 cells were exposed to vehicle control, 10 μM p-XSC, or 100 μM p-XSeSG in fresh media. After 18 hours of drug exposure, cell extracts were assayed for luciferase activity and GSH levels and nuclear extracts were analyzed for Nrf2 by Western blotting using Lamin A as a control marker. Data are presented as mean ± SD (n=3).