. 2012 Oct 17;103(8):1706–1718. doi: 10.1016/j.bpj.2012.09.001

Table 1.

Functional properties of inter-CBS linker domain mutants

	Current @−140 mV (pA/pF)		Activation voltage (mV)		50% rise time (ms)
Mutant	−GCK-3	+GCK-3	−GCK-3	+GCK-3	−GCK-3	+GCK-3
Δ628-647	−360.4 ± 26.2	−167.2 ± 27.6	−40.0 ± 1.1	−67.2 ± 2.1	4.1 ± 0.9	74.7 ± 14.8
Δ628-647	−360.4 ± 26.2	P < 0.0015	−40.0 ± 1.1	P < 0.0001	4.1 ± 0.9	P < 0.02
Δ648-667	−403.9 ± 39.5	−108.5 ± 13.2	−36.2 ± 0.8	−60.5 ± 1.7	2.2 ± 0.2	32.6 ± 12.8
Δ648-667	−403.9 ± 39.5	P < 0.0004	−36.2 ± 0.8	P < 0.0001	2.2 ± 0.2	P < 0.05
Ala-636-647	−318.9 ± 48.8	−122.4 ± 14.8	−36.5 ± 0.8	−58.5 ± 1.1	3.0 ± 0.2	59.1 ± 5.3
Ala-636-647	−318.9 ± 48.8	P < 0.0001	−36.5 ± 0.8	P < 0.0001	3.0 ± 0.2	P < 0.0001
Δ668-687	−299.0 ± 42.4	−406.8 ± 23.3	−35.7 ± 0.6	−35.4 ± 1.3	2.7 ± 0.2	2.7 ± 0.4
Δ668-687	−299.0 ± 42.4	P > 0.1	−35.7 ± 0.6	P > 0.8	2.7 ± 0.2	P > 0.99
Δ688-709	−362.3 ± 14.8	−382.3 ± 52.3	−33.8 ± 0.7	−34.5 ± 0.4	1.8 ± 0.3	1.9 ± 0.1
Δ688-709	−362.3 ± 14.8	P > 0.7	−33.8 ± 0.7	P > 0.4	1.8 ± 0.3	P > 0.8
Δ688-709/S742E/S747E	−223.1 ± 27.1^∗		−43.7 ± 1.1^†		11.2 ± 2.5^‡
Δ708-727	−404.9 ± 43.8	−211.6 ± 36.5	−34.7 ± 0.4	−57.2 ± 1.3	2.2 ± 0.2	29.2 ± 7.5
Δ708-727	−404.9 ± 43.8	P < 0.01	−34.7 ± 0.4	P < 0.0001	2.2 ± 0.2	P < 0.01
Δ769-777	−344.0 ± 34.9	−66.7 ± 10.7	−45.6 ± 1.1	−65.5 ± 0.3	15.6 ± 2.7	79.4 ± 5.3
Δ769-777	−344.0 ± 34.9	P < 0.001	−45.6 ± 1.1	P < 0.0001	15.6 ± 2.7	P < 0.0001
Δ728-737	−154.2 ± 34.5	−60.0 ± 12.8	−48.2 ± 1.0	−66.7 ± 0.8	15.1 ± 2.4	56.3 ± 10.2
Δ728-737	−154.2 ± 34.5	P < 0.04	−48.2 ± 1.0	P < 0.0001	15.1 ± 2.4	P < 0.01
Δ752-759	−134.3 ± 23.0	−49.9 ± 14.5	−43.5 ± 1.1	−70.8 ± 1.6	7.8 ± 1.9	51.1 ± 7.5
Δ752-759	−134.3 ± 23.0	P < 0.02	−43.5 ± 1.1	P < 0.0001	7.8 ± 1.9	P < 0.005
Δ760-768	−195.0 ± 24.0	−91.1 ± 6.0	−45.2 ± 3.5	−62.8 ± 2.9	6.2 ± 1.8	66.0 ± 5.6
Δ760-768	−195.0 ± 24.0	P < 0.0007	−45.2 ± 3.5	P < 0.008	6.2 ± 1.8	P < 0.0002

Values are mean ± SE (n = 3–12). Channels were coexpressed with either kinase dead GCK-3 (−GCK-3) or WT GCK-3 (+GCK-3).

^∗P < 0.005, ^†P < 0.0001, and ^‡P < 0.02, and compared to Δ688-709 mutant. P values shown for all other mutants were generated by comparing to the mutant channel expressed with kinase dead GCK-3.