. 2012 Oct 17;103(8):1706–1718. doi: 10.1016/j.bpj.2012.09.001

Table 2.

Functional properties of channels with mutations in the H-I loop, R-helix linker, D-helix, and α1 of CBS2

	Current @−140 mV (pA/pF)		Activation voltage (mV)		50% rise time (ms)
Mutant	−GCK-3	+GCK-3	−GCK-3	+GCK-3	−GCK-3	+GCK-3
V231A	−392.2 ± 12.6	−125.4 ± 43.5	−36.3 ± 1.5	−66.7 ± 1.1	4.0 ± 0.6	94.6 ± 6.2
V231A	−392.2 ± 12.6	P < 0.004	−36.3 ± 1.5	P < 0.0001	4.0 ± 0.6	P < 0.0007
F233A	−248.0 ± 36.0	−133.9 ± 20.0	−42.2 ± 2.8	−69.1 ± 1.1	2.6 ± 0.7	12.3 ± 4.1
F233A	−248.0 ± 36.0	P < 0.01	−42.2 ± 2.8	P < 0.0001	2.6 ± 0.7	P < 0.03
Y540A	−126.9 ± 18.1	−61.3 ± 7.9	−48.6 ± 0.8	−84.9 ± 3.5	4.0 ± 0.4	22.6 ± 2.7
Y540A	−126.9 ± 18.1	P < 0.006	−48.6 ± 0.8	P < 0.0001	4.0 ± 0.4	P < 0.0001
L541A	−171.7 ± 21.2	−83.1 ± 13.0	−43.5 ± 1.4	−84.0 ± 2.9	2.9 ± 0.4	9.2 ± 1.7
L541A	−171.7 ± 21.2	P < 0.0012	−43.5 ± 1.4	P < 0.0001	2.9 ± 0.4	P < 0.01
P542A	−215.3± 28.6	−47.2 ± 16.2	−36.6 ± 2.7	−62.1 ± 2.9	1.7 ± 0.2	20.7 ± 3.4
P542A	−215.3± 28.6	P < 0.0002	−36.6 ± 2.7	P < 0.0001	1.7 ± 0.2	P < 0.0008
R134A	−158.3 ± 20.8	−32.1 ± 7.2	−29.4 ± 0.5	−74.8 ± 2.3	1.5 ± 0.2	27.0 ± 8.0
R134A	−158.3 ± 20.8	P < 0.0007	−29.4 ± 0.5	P < 0.0001	1.5 ± 0.2	P < 0.013
S809A	−191.3 ± 67.3	−51.1 ± 13.7	−33.0 ± 1.1	−65.4 ± 4.0	3.1 ± 0.5	36.4 ± 8.5
S809A	−191.3 ± 67.3	P < 0.03	−33.0 ± 1.1	P < 0.001	3.1 ± 0.5	P < 0.02
L810A	−290.8 ± 43.7	−342.3 ± 63.1	−31.6 ± 0.9	−49.0 ± 1.1	5.3 ± 0.4	17.5 ± 2.0
L810A	−290.8 ± 43.7	P > 0.5	−31.6 ± 0.9	P < 0.0001	5.3 ± 0.4	P < 0.0001

Values are mean ± SE (n = 3–10). Channels were coexpressed with either kinase dead GCK-3 (−GCK-3) or WT GCK-3 (+GCK-3). P values shown were generated by comparing to the mutant channel expressed with kinase dead GCK-3.