Figure 3.

(A) Turning and elongation of an axon in the netrin-1 gradient. Scale bar: 10 μm. (B) Turning angles show a significant repulsive effect in the netrin gradient (mean ± SE = −19 ± 5°, 18 cells, gray bar) compared to control conditions using Rhodamine-labeled dextran (2 ± 6°, 17 cells, white bar). Kolmogorov-Smirnov test, p = 0.007. (C) The netrin gradient has no significant effect on the growth speed (0.67 ± 0.12 μm.min⁻¹) compared to control conditions (0.74 ± 0.06 μm.min⁻¹). (D) Example of trajectories of individual GCs in the Netrin-1 gradient.