Figure 4.

Superresolution fluorescence imaging. (a) Microtubules at the bottom of COS-7 were imaged via dSTORM. (Inset) Comparison between dSTORM (left) and conventional fluorescence microscopy (right). (b) Microtubule width in the dSTORM image. The cross-section profile of the microtubule (right) was generated by collecting a number of localization spots along the line perpendicular to the length of the microtubule in the white box in the dSTORM image. The histogram was well fitted to a Gaussian function with FWHM of 59 nm (right, blue lines). (c–e) Superresolution images at depths of 4 μm (c), 7.5 μm (d), and 85 μm (e), respectively. The image in e was obtained from a cell fixed on the glass slide side of the channel using the water-immersion objective. For all other experiments, the oil-immersion lens was used. Exposure time, 100 ms.