RMSD along sequence for the free (red circles and lines) and substrate-bound (blue squares and lines) forms of TEM-1 (A) and PSE-4 (B). Catalytic residues (S70, K73, S130, E166, and K/R234) are indicated with gray lines, the Ω loop with a light gray box. Above panels are TEM-1 and PSE-4 sequences: α-helices are white boxes; β-strands gray boxes; the Ω loop is in light gray; a vertical bar in H2 indicates the bend between H2A and H2B.