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ACF- and CCF-calculated velocity maps of CHO.B2 α6β1-GFP and paxillin-mKO superimposed on the first image from the TOI. Nf = 10, frames 71–80, δt = 5 s, px,y = 0.105 μm, and 16 × 16 pixels (1.7 × 1.7 μm) ROI (yellow square). Representative of six adhesions measured in six cells.
