Figure 5.

Zebrafish Barhl2 morpholino causes efficient barhl2 knockdown. A, A translational blocking MO was designed against 6 base pairs upstream the translational start site of zebrafish barhl2 mRNA. The morpholino efficiency was tested using anti-Barhl2 antibody. B, Western Blotting of wild-type (control) and morphant (0.5 and 1 mm Barhl2 MO) 50-hpf-old embryos heads. C, Micrographs of control and Barhl2 morphant embryos. Morphants look relatively normal, showing only mild general defects in their heads. D–G, Immunohistochemical labeling of brain (D, E) and retinal (F, F′, G) sections at 50 hpf. Control embryos (D, F) and standard MO-injected embryos (F′) show Barhl2 protein in the diencephalon region D around the ventricular zone V of the brain (D) and in the retina (F, F′), in ACs and HCs and in cells located in the ganglion cell layer (GCL; F, F′). Barhl2 morphant embryo brain and retina do not stain for Barhl2 protein. Scale bar: (in G) D–G, 50 μm.