Fig. 4.

A truncated TCRγ chain lacking both extracellular Ig-like domains can pair with pTα to initiate signaling. Graphical representation of the percentages of CD4⁺CD8⁺ DP cells generated when (A) E14 RAG-2^−/− (n = 3 experiments) or (B) [pTα^−/−.TCRδ^−/−] thymocytes (n = 3 experiments) were transduced with retroviral vector expressing GFP alone (vector control) or with retroviral vectors expressing GFP and TCRΔIIγ, TCRΔIIδ, or both, as indicated, and cultured on OP9-DL1 stromal cells for 7 days. Mock cells received no virus. Black bars represent GFP⁻ cells from the culture, whereas white bars represent GFP⁺ cells. **, P ≤ 0.002; ***, P ≤ 0.0005; ns = not significant.