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. 2012 Aug 29;32(35):12051–12065. doi: 10.1523/JNEUROSCI.1857-12.2012

Figure 3.

Figure 3.

Conditional dab1 deletion induces aberrant DGC migration. A, C, NestinCreERT2/Dab1+/+ mice treated with TMX at P7–P8 had properly located neuroblasts in the dentate subgranular zone and inner granule cell layer (gcl) as assessed by PSA-NCAM immunoreactivity 52 d later. B, D, In NestinCreERT2/Dab1flox/flox mice treated with the same TMX regimen, PSA-NCAM immunolabeling was more disorganized, and many PSA-NCAM-positive cells appeared in the hilus (h). In addition, chains or clusters of presumptive migrating neuroblasts extended from the gcl into the h (D, arrowheads). Scale bar: (in A), A, B, 50 μm; (in A) C, D, 35 μm. E, F, GFAP immunostaining of DG from P60 NestinCreERT2/Dab1+/+ control (E) and NestinCreERT2/Dab1flox/flox mice (F) treated with TMX at P7–P8. Labeled glia, including radial glia-like cells in the gcl, appeared unaffected by conditional loss of dab1. Scale bars: (in A) A, B, 50 μm; C, D, 35 μm; (in E) E, F, 150 μm.