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. Author manuscript; available in PMC: 2013 Oct 3.
Published in final edited form as: Cell Metab. 2012 Oct 3;16(4):487–499. doi: 10.1016/j.cmet.2012.09.004

Figure 7.

Figure 7

Silencing of XBP1 mRNA in vivo. (A) Female C57BL/6 mice were i.v. injected with PBS, luciferase siRNA (5 mg/kg), or varying doses of XBP1 siRNA. Mice were sacrificed 48 hrs later. Hepatic XBP1 mRNA levels were measured by qRT-PCR. n = 4 mice per group. Values represent mean ± SEM. ***P <0.001 compared with the control groups (PBS and luciferase siRNA groups). (B) Western blot to measure XBP1s protein levels in pooled nuclear extracts. *non-specific band. (C) Mice were injected with siRNAs at 7.5 mg/kg, and sacrificed after indicated days to measure hepatic XBP1s protein levels by western blot. SP1 serves as a loading control. n = 3–4 mice per group. (D) qRT-PCR analysis of hepatic gene expression. n = 3 per group. *P <0.05 (E) Western blot analysis of IRE1α in liver lysates. The panel shows IRE1α phosphorylation measured by Phos-Tag western blot. (F–G) Male C57BL/6, or (H–I) Apoe−/− mice were i.v. injected with siRNAs (7.5 mg/kg), and bled to measure plasma lipid levels. n = 5 –8 per group.. Values represent mean ± SEM. *P <0.05, **P <0.01, ***P <0.001 compared with the control group (siLuc).