Table 1. Primers used in RT–PCR for neural markers.
Gene | Accession no. | Primer sequence (5′–3′) | Size (bp) | Annealing (°C) |
---|---|---|---|---|
Nestin | NM_006617 | F:AGCAGCACTCTTAACTTACG | 259 | 55 |
R:CTGACTTAGCCTATGAGATGG | ||||
Vimentin | NM_003380 | F:GCTCAGATTCAGGAACAG | 621 | 55 |
R:GCAGGTCTTGGTATTCAC | ||||
Map2 | NM_002374 | F:TGAAGAATGGCAGATGAAC | 214 | 56 |
R:AGAAGGAGGCAGATTAGC | ||||
Olig1 | NM_138983 | F:TAACCAGGCGTCTCACAG | 347 | 55 |
R:ATTCGGCTACTACCAACAAC | ||||
GFAP | NM_002055 | F:GAATGAGGAGGAAGGAGAG | 282 | 57 |
R:CCAGGAGTTCAAGGTCAG | ||||
GABA | NM_001182 | F:TAACAACGAGCCAATAGC | 475 | 55 |
R:GACAGCCACACTAATGAG | ||||
β-actin | NM_001101 | F:CGTGACATTAAGGAGAAG | 202 | 56 |
R:TGATGGAGTTGAAGGTAG |