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. 2012 Apr 26;19(1):e00015. doi: 10.1042/CBR20110009

Table 1. Primers used in RT–PCR for neural markers.

Gene Accession no. Primer sequence (5′–3′) Size (bp) Annealing (°C)
Nestin NM_006617 F:AGCAGCACTCTTAACTTACG 259 55
R:CTGACTTAGCCTATGAGATGG
Vimentin NM_003380 F:GCTCAGATTCAGGAACAG 621 55
R:GCAGGTCTTGGTATTCAC
Map2 NM_002374 F:TGAAGAATGGCAGATGAAC 214 56
R:AGAAGGAGGCAGATTAGC
Olig1 NM_138983 F:TAACCAGGCGTCTCACAG 347 55
R:ATTCGGCTACTACCAACAAC
GFAP NM_002055 F:GAATGAGGAGGAAGGAGAG 282 57
R:CCAGGAGTTCAAGGTCAG
GABA NM_001182 F:TAACAACGAGCCAATAGC 475 55
R:GACAGCCACACTAATGAG
β-actin NM_001101 F:CGTGACATTAAGGAGAAG 202 56
R:TGATGGAGTTGAAGGTAG