Figure 9. Expression of enzymes involved in ROS detoxification after exposure to insulin or palmitate.

C2C12 myotubes were either left untreated or treated with either 100 nM insulin or palmitate (0.2 mM, conjugated to BSA 4.8%) for 24 h. The mRNA abundance of mitochondrial genes was determined by quantitative real-time PCR by ΔΔ^C_T method. Data are means±S.E.M. for n=3 experiments, quantified in triplicate. Statistical analysis: ANOVA (*P<0.05).