Table 1. Spectral features of sAC-HD variants with exchanges at positions potentially acting as axial haem ligands.
ox., oxidized; red., reduced; wt, wild-type.
| Protein variant | Soret ox. (nm) | Soret red. (nm) | Soret ox.+NO (nm) | Soret red.+NO (nm) |
|---|---|---|---|---|
| wt | 414 | 424 | 409 | 417 |
| H950A | 421 | 426 | 398 | 425 |
| H962A | 420, 422 | 425, 425 | 394, 401 | −404 |
| H966A | 417, 422 | 425, 425 | 394, 401 | −404 |
| H976A | 412, 413 | 420, 426 | 397, 399 | 425 |
| H977A* | − | − | − | − |
| H999A | 420, 421 | 425, 426 | 394, 402 | −402 |
| CDHCA | 419 | 426 | 399 | 425 |
| CDHCH | 421 | 426 | 400 | 401 |
*No protein expression was observed.